| Sunday, 20 September 2009 |
| Lichfield Ladies | 44-3 | Bristol Ladies |
| Richmond Ladies | 36-0 | Wasps Ladies |
| Saracen Ladies | 28-7 | Team Northumbria Ladies |
| Worcester Ladies | 15-13 | Darlington Mowden P Ladies |
| Sunday, 04 October 2009 |
| Darlington Mowden P Ladies | 36-17 | Bristol Ladies |
| Team Northumbria Ladies | 5-29 | Richmond Ladies |
| Wasps Ladies | 22-12 | Lichfield Ladies |
| Worcester Ladies | 7-15 | Saracen Ladies |
| Sunday, 11 October 2009 |
| Bristol Ladies | 3-24 | Wasps Ladies |
| Lichfield Ladies | 27-5 | Team Northunbria Ladies |
| Richmond Ladies | 34-5 | Worcester Ladies |
| Saracen Ladies | 47-3 | Darlington Mowden P Ladies |
| Sunday, 25 October 2009 |
| Saracen Ladies | 13-13 | Richmond Ladies |
| Team Northumbria Ladies | 5-24 | Bristol Ladies |
| Wasps Ladies | 26-0 | Darlington Mowden P Ladies |
| Worcester Ladies | 20-0 | Lichfield Ladies |
| Sunday, 01 November 2009 |
| Bristol Ladies | 26-12 | Worcester Ladies |
| Lichfield Ladies | 0-15 | Saracen Ladies |
| Richmond Ladies | 63-0 | Darlington Mowden P Ladies |
| Wasps Ladies | 31-12 | Team Northumbria Ladies |
| Sunday, 08 November 2009 |
| Darlington Mowden P Ladies | P | Team Northumbria Ladies |
| Richmond Ladies | P | Lichfield Ladies |
| Saracen Ladies | P | Bristol Ladies |
| Worcester Ladies | P | Wasps Ladies |
| Sunday, 29 November 2009 |
| Bristol Ladies | V | Richmond Ladies |
| Lichfield Ladies | V | Darlington Mowden P Ladies |
| Saracen Ladies | V | Wasps Ladies |
| Team Northumbria Ladies | V | Worcester Ladies |
| Sunday, 06 December 2009 |
| Bristol Ladies | V | Darlington Mowden P Ladies |
| Lichfield Ladies | V | Wasps Ladies |
| Richmond Ladies | V | Team Northumbria Ladies |
| Saracen Ladies | V | Worcester Ladies |
| Sunday, 13 December 2009 |
| Darlington Mowden P Ladies | V | Saracen Ladies |
| Team Northumbria Ladies | V | Lichfield Ladies |
| Wasps Ladies | V | Bristol Ladies |
| Worcester Ladies | V | Richmond Ladies |
| Sunday, 10 January 2010 |
| Bristol Ladies | V | Team Northumbria Ladies |
| Darlington Mowden P Ladies | V | Wasps Ladies |
| Lichfield Ladies | V | Worcester Ladies |
| Richmond Ladies | V | Saracen |
| Sunday, 17 January 2010 |
| Darlington Mowden P Ladies | V | Richmond Ladies |
| Saracen Ladies | V | Lichfield Ladies |
| Team Northumbria Ladies | V | Wasps Ladies |
| Worcester Ladies | V | Bristol Ladies |
| Sunday, 24 January 2010 |
| Bristol Ladies | V | Saracen Ladies |
| Lichfield Ladies | V | Richmond Ladies |
| Team Northumbria Ladies | V | Darlington Mowden P Ladies |
| Wasps Ladies | V | Worcester Ladies |
| Saturday, 27 March 2010 |
| Wasps Ladies | V | Saracen Ladies |
| Sunday, 28 March 2010 |
| Darlington Mowden P Ladies | V | Lichfield Ladies |
| Richmond Ladies | V | Bristol Ladies |
| Worcester Ladies | V | Team Northumbria Ladies |
| Sunday, 11 April 2010 |
| Bristol Ladies | V | Lichfield Ladies |
| Darlington Mowden P Ladies | V | Worcester Ladies |
| Team Northumbria Ladies | V | Saracen Ladies |
| Wasps Ladies | V | Richmond Ladies |